By Sterling Chaykin (auth.), Seymour Rothchild (eds.)
Radioactive tracers have had a profound impression on medical study in past times two decades, fairly in bio chemistry and allied fields. The quick evolution of refined instrumentation and labeling recommendations in the past ten years has made attainable on a regimen foundation exquisitly delicate experiments which with different strategies will require her culean efforts at the a part of the investigator. The Symposia on Advances in Tracer method, that have been held on a regular basis due to the fact that 1957, supply for examine employees in biochemistry, pharmacology, endocrinology, and drugs a spotlight at the various advancements within the tracer box which are often scattered in the course of the clinical literature. quantity three is arecord of the complaints of the 9th and 10th Symposia, that have been subsidized by means of the recent England Nuclear company and the Packard software corporation, Inc. contemporary advances in tracer method, as mirrored through the papers during this quantity, were quite often involved in im proved analytical concepts, reminiscent of liquid scintillation count number ing, autoradiography, and chromatography, and with novel ap plications of classified compounds in biochemical and scientific problems.
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Additional resources for Advances in Tracer Methodology: Volume 3 A collection of papers presented at the Ninth and Tenth Symposia on Tracer Methology
1. Method A. this case besides the formation of acrylic acid that of a radioactive carboxylic acid of greater retention time. The preparation of a vinyl-halide-free Grignard reagent led us: 1. To prefer the use of vinyl bromide, because it reacts with magnesium more easily and rapidly than the chloride. Furthermore, it is not necessary to induce the reaction by the addition of ethyl bromide. 2. To measure accurately the quantity of vinyl bromide made to react with an excess of magnesium. Under these conditions, the yield of magnesium reagent is around 90%.
Proc. 23:380 (1964). , j. Org. Chern. 23:1037 (1963). , and Kernp, R. , j. Biol. Chern. ,. j. Biol. , August 1966. 21 (1965). (1963). (1963). (1965). USE OF VINYL MAGNESIUM BROMIDE IN THE SYNTHESIS OF LABELED COMPOUNDS: NEW PREPARATIONS OF GLYCEROL-I-C14 AND GLYCERIC ACID-I_C14 L. Pichat, M. Herbert, and F. P. n° 2 GiF /Yvette (91), France The biological importance of glycerol and glyceric acid is well known; as a consequence, it is very useful to have methods for the labeling of these compounds with C14.
6 6-aminopurine (adenine) . •• 110 mc mc mc mc mc per per per per per millimole millimole millimole millimole milli mole We observe in this series some conspicuous peculiarities. First. one would expect the substituent-free purine to show the highest tritium incorporation because of the highest number of CH-groups which can be labeled. Actually. however, the individual substituents affect the basic structure, causing a decrease or increase of substitution which is dependent on the site of the substitution.
Advances in Tracer Methodology: Volume 3 A collection of papers presented at the Ninth and Tenth Symposia on Tracer Methology by Sterling Chaykin (auth.), Seymour Rothchild (eds.)